A nitrate-permeable ion channel in the tonoplast of the moss Physcomitrella patens.

ABSTRACT:

Main conclusion

In this work, for the first time the activity of nitrate-permeable channels in the tonoplast of the moss Physcomitrella patens was recorded. The channels allowed nitrate flow in one direction-from the cytoplasm to the vacuole. Selectivity of nitrate over chloride of the channels was proved. The activity of the channels was dependent on cytoplasmic calcium, magnesium, and pH. A patch-clamp study carried out on the vacuolar membrane of the moss Physcomitrella patens has revealed that inhibition of cation-selective channels leads to disclosure of channels permeable to NO3 (-). These channels were inwardly rectifying and allowed anions to flow from the cytoplasm to the vacuole. After a decrease in the cytoplasmic NO3 (-) concentration, the current density recorded in th

SUBMITTER: Koselski M 

PROVIDER: S-EPMC4412613 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature