Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.

ABSTRACT: A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10 min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.

SUBMITTER: Wang L 

PROVIDER: S-EPMC4412846 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature