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Functional recombinants designed from a fetuin/asialofetuin-specific marine algal lectin, rhodobindin.


ABSTRACT: Plant lectins have attracted much attention for biomedical applications including targeted drug delivery system and therapy against tumors and microbial infections. The main problem of using lectins as a biomedical tool is a batch-to-batch variation in isoforms content. The production of lectins using recombination tools has the advantage of obtaining high amounts of proteins with more precise properties, but there are only a handful of functional recombinant lectins presently available. A fetuin/asialo-fetuin specific lectin, Rhodobindin, has unique tandem repeats structure which makes it useful in exploiting for recombinant lectin. We developed three functional recombinant lectins using E. coli expression system: one from full cDNA sequence and two from fragmentary sequences of Rhodobindin. Hemagglutinating activity and solubility of the recombinant lectins were highest at OD 0.7 cell concentration at 20 °C. The optimized process developed in this study was suitable for the quality-controlled production of high amounts of soluble recombinant lectins.

SUBMITTER: Han JW 

PROVIDER: S-EPMC4413206 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Functional recombinants designed from a fetuin/asialofetuin-specific marine algal lectin, rhodobindin.

Han Jong Won JW   Jung Min Gui MG   Shim Eun Young EY   Shim Jun Bo JB   Kim Young Min YM   Kim Gwang Hoon GH  

Marine drugs 20150413 4


Plant lectins have attracted much attention for biomedical applications including targeted drug delivery system and therapy against tumors and microbial infections. The main problem of using lectins as a biomedical tool is a batch-to-batch variation in isoforms content. The production of lectins using recombination tools has the advantage of obtaining high amounts of proteins with more precise properties, but there are only a handful of functional recombinant lectins presently available. A fetui  ...[more]

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