Productive mRNA stem loop-mediated transcriptional slippage: Crucial features in common with intrinsic terminators.

ABSTRACT: Escherichia coli and yeast DNA-dependent RNA polymerases are shown to mediate efficient nascent transcript stem loop formation-dependent RNA-DNA hybrid realignment. The realignment was discovered on the heteropolymeric sequence T5C5 and yields transcripts lacking a C residue within a corresponding U5C4. The sequence studied is derived from a Roseiflexus insertion sequence (IS) element where the resulting transcriptional slippage is required for transposase synthesis. The stability of the RNA structure, the proximity of the stem loop to the slippage site, the length and composition of the slippage site motif, and the identity of its 3' adjacent nucleotides (nt) are crucial for transcripts lacking a single C. In many respects, the RNA structure requirements for this slippage resemble those f

SUBMITTER: Penno C 

PROVIDER: S-EPMC4413344 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature