Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production.

ABSTRACT:

Background

Terpenoids (isoprenoids) have numerous applications in flavors, fragrances, drugs and biofuels. The number of microbially produced terpenoids is increasing as new biosynthetic pathways are being elucidated. However, efforts to improve terpenoid production in yeast have mostly taken advantage of existing knowledge of the sterol biosynthetic pathway, while many additional factors may affect the output of the engineered system.

Results

Aiming to develop a yeast strain that can support high titers of sclareol, a diterpene of great importance for the perfume industry, we sought to identify gene deletions that improved carotenoid, and thus potentially sclareol, production. Using a carotenogenic screen, the best 100 deletion mutants, out of 4,700 mutant strains, were sel

SUBMITTER: Trikka FA 

PROVIDER: S-EPMC4413541 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature