Temporal genome expression profile analysis during t-cell-mediated colitis: identification of novel targets and pathways.

ABSTRACT:

Background

T cells critically regulate inflammatory bowel disease (IBD), with T-cell-dependent experimental colitis models gaining favor in identifying potential pathogenic mechanisms; yet limited understanding of specific pathogenic molecules or pathways still exists.

Methods

In this study we sought to identify changes in whole genome expression profiles using the CD4CD45Rbhi T-cell transfer colitis model compared to genome expression differences from Crohn's disease (CD) tissue specimens. Colon tissue was used for histopathological and genome expression profiling analysis at 0, 2, 4, or 6 weeks after adoptive T-cell transfer.

Results

We identified 1775 genes that were significantly altered during disease progression, with 361 being progressively downregulated and 34

SUBMITTER: Fang K 

PROVIDER: S-EPMC4413946 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature