An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation.

ABSTRACT:

Background

The gene encoding the serine biosynthesis pathway enzyme PHGDH is located in a region of focal genomic copy number gain in human cancers. Cells with PHGDH amplification are dependent on enzyme expression for proliferation. However, dependence on increased PHGDH expression extends beyond production of serine alone, and further studies of PHGDH function are necessary to elucidate its role in cancer cells. These studies will require a physiologically relevant form of the enzyme for experiments using engineered cell lines and recombinant protein.

Results

The addition of an N-terminal epitope tag to PHGDH abolished the ability to support proliferation of PHGDH-amplified cells despite retention of some activity to convert 3-PG to PHP. Introducing an R236E mutation into

SUBMITTER: Mattaini KR 

PROVIDER: S-EPMC4414297 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature