The transcription map of HPV11 in U2OS cells adequately reflects the initial and stable replication phases of the viral genome.

ABSTRACT:

Background

Although prophylactic vaccines have been developed against HPV6, HPV11, HPV16 and HPV18 there is the clear unmet medical need in order to justify the development of drugs targeting human papillomavirus replication. The native host cells of HPVs are human primary keratinocytes which can be cultivated in raft cultures. However, this method is difficult to use in high-throughput screening assays and the need for a cost-effective cellular system for screening potential anti-HPV drug candidates during all stages of HPV genome replication remains.

Methods

U2OS cells were transfected with HPV11 wt or E8- minicircle genomes and their gene expression was studied via 3' RACE, 5' RACE or via real time PCR methods. The DNA replication of these genomes was detected by Southern

SUBMITTER: Isok-Paas H 

PROVIDER: S-EPMC4414447 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature