Inflammation and the depot-specific secretome of human preadipocytes.

ABSTRACT:

Objective

Visceral white adipose tissue (WAT) expansion and macrophage accumulation are associated with metabolic dysfunction. Visceral WAT typically shows greater macrophage infiltration. Preadipocytes show varying proinflammatory expression profiles among WAT depots. The objective was to examine the secretomes and chemoattractive properties of preadipocytes from visceral and subcutaneous WAT.

Methods

A label-free quantitative proteomics approach was applied to study secretomes of subcutaneous and omental preadipocytes from obese subjects. Enzyme-linked immunosorbent assays and chemotaxis assays were used to confirm proinflammatory chemokine secretion between depots.

Results

Preadipocyte secretomes showed greater variation between depots than did intracellular protei

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC4414793 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature