Ontology highlight
ABSTRACT:
SUBMITTER: Liao J
PROVIDER: S-EPMC4414868 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Nature genetics 20150330 5
DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases (DNMTs) in human embryonic stem cells (ESCs) using CRISPR/Cas9 genome editing to further investigate the roles and genomic targets of these enzymes. Disruption of DNMT3A or DNMT3B individually as well as of both enzymes in tandem results in viable, pluripotent cell lines with distinct effects on the DN ...[more]