Multiple transcripts of anoctamin genes expressed in the mouse submandibular salivary gland.

ABSTRACT:

Purpose

Salivary fluid formation is primarily driven by Ca(2+)-activated, apical efflux of chloride into the lumen of the salivary acinus. The anoctamin1 protein is an anion channel with properties resembling the endogenous calcium-activated chloride channels. In order to better understand the role of anoctamin proteins in salivary exocrine secretion, the expression of the ten members of the anoctamin gene family in the mouse submandibular gland was studied.

Methods

Total RNA extracted from mouse submandibular salivary glands was reverse transcribed using primer pairs to amplify the full-length coding regions of each anoctamin gene and was subcloned into plasmid vectors for DNA sequencing. Alternative splice variants were also screened by polymerase chain reaction using prim

SUBMITTER: Han JH 

PROVIDER: S-EPMC4415004 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature