A systems biology approach identifies a regulatory network in parotid acinar cell terminal differentiation.

ABSTRACT:

Objective

The transcription factor networks that drive parotid salivary gland progenitor cells to terminally differentiate, remain largely unknown and are vital to understanding the regeneration process.

Methodology

A systems biology approach was taken to measure mRNA and microRNA expression in vivo across acinar cell terminal differentiation in the rat parotid salivary gland. Laser capture microdissection (LCM) was used to specifically isolate acinar cell RNA at times spanning the month-long period of parotid differentiation.

Results

Clustering of microarray measurements suggests that expression occurs in four stages. mRNA expression patterns suggest a novel role for Pparg which is transiently increased during mid postnatal differentiation in concert with several tar

SUBMITTER: Metzler MA 

PROVIDER: S-EPMC4416001 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature