A novel assay for the detection of bioactive volatiles evaluated by screening of lichen-associated bacteria.

ABSTRACT: Volatile organic compounds (VOCs) produced by microorganisms are known both for their effect on pathogens and their role as mediators in various interactions and communications. Previous studies have demonstrated the importance of VOCs for ecosystem functioning as well as their biotechnological potential, but screening for bioactive volatiles remained difficult. We have developed an efficient testing assay that is based on two multi-well plates, separated by a sealing silicone membrane, two tightening clamps, and variable growth media, or indicators. The experiment design as presented here is a novel and robust technique to identify positive as well as negative VOC effects on the growth of a target organism and to test for specific substances e.g., hydrogen cyanide which can be detected wi

SUBMITTER: Cernava T 

PROVIDER: S-EPMC4416446 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature