RNA polymerase II pausing can be retained or acquired during activation of genes involved in the epithelial to mesenchymal transition.

ABSTRACT: Promoter-proximal RNA polymerase II (Pol II) pausing is implicated in the regulation of gene transcription. However, the mechanisms of pausing including its dynamics during transcriptional responses remain to be fully understood. We performed global analysis of short capped RNAs and Pol II Chromatin Immunoprecipitation sequencing in MCF-7 breast cancer cells to map Pol II pausing across the genome, and used permanganate footprinting to specifically follow pausing during transcriptional activation of several genes involved in the epithelial to mesenchymal transition (EMT). We find that the gene for EMT master regulator Snail (SNAI1), but not Slug (SNAI2), shows evidence of Pol II pausing before activation. Transcriptional activation of the paused SNAI1 gene is accompanied by a further incre

SUBMITTER: Samarakkody A 

PROVIDER: S-EPMC4417172 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature