Engineering Saccharomyces pastorianus for the co-utilisation of xylose and cellulose from biomass.

ABSTRACT:

Background

Lignocellulosic biomass is a viable source of renewable energy for bioethanol production. For the efficient conversion of biomass into bioethanol, it is essential that sugars from both the cellulose and hemicellulose fractions of lignocellulose be utilised.

Results

We describe the development of a recombinant yeast system for the fermentation of cellulose and xylose, the most abundant pentose sugar in the hemicellulose fraction of biomass. The brewer's yeast Saccharomyces pastorianus was chosen as a host as significantly higher recombinant enzyme activities are achieved, when compared to the more commonly used S. cerevisiae. When expressed in S. pastorianus, the Trichoderma reesei xylose oxidoreductase pathway was more efficient at alcohol production from xylose t

SUBMITTER: Kricka W 

PROVIDER: S-EPMC4417197 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature