Evaluation of reference genes for insect olfaction studies.

ABSTRACT:

Background

Quantitative reverse transcription PCR (qRT-PCR) is a robust and accessible method to assay gene expression and to infer gene regulation. Being a chain of procedures, this technique is subject to systematic error due to biological and technical limitations mainly set by the starting material and downstream procedures. Thus, rigorous data normalization is critical to grant reliability and repeatability of gene expression quantification by qRT-PCR. A number of 'housekeeping genes', involved in basic cellular functions, have been commonly used as internal controls for this normalization process. However, these genes could themselves be regulated and must therefore be tested a priori.

Methods

We evaluated eight potential reference genes for their stability as internal

SUBMITTER: Omondi BA 

PROVIDER: S-EPMC4417234 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature