A microRNA profile associated with Opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma.

ABSTRACT:

Background

Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive tumor of the bile duct, and a significant public health problem in East Asia, where it is associated with infection by the parasite Opisthorchis viverrini. ICC is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority.

Methods

We have comprehensively profiled miRNA expression levels in ICC tumor tissue using small RNA-Seq and validated these profiles using quantitative PCR on matched plasma samples.

Results

Distinct miRNA profiles were associated with increasing histological differentiation of ICC tumor tissue. We also observed that histologically normal tissue adjacent to ICC tumor displayed miRNA expression profiles more similar to tumor

SUBMITTER: Plieskatt J 

PROVIDER: S-EPMC4417245 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature