Amnion cell-mediated immune modulation following bleomycin challenge: controlling the regulatory T cell response.

ABSTRACT:

Introduction

The immunomodulatory properties of human amnion epithelial cells (hAECs) have been previously described in several disease models. We previously reported on the ability of hAECs to influence macrophage phenotype and chemotaxis. In this study, we aim to elucidate the contribution of regulatory T cells (Tregs) to macrophage polarisation and downstream effects on inflammation and fibrosis in a bleomycin model of lung injury.

Methods

Either CD45(+)/FoxP3(+) Tregs or CD45(+)/FoxP3 (-) non-Tregs were adoptively transferred into Rag1 (-/-) mice immediately prior to bleomycin challenge. Four million hAECs were administered 24 hours later. Outcomes were measured 7 or 14 days later.

Results

Mitigation of lung inflammation and fibrosis was observed only in animals t

SUBMITTER: Tan JL 

PROVIDER: S-EPMC4417266 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature