Molecular differences between stromal cell populations from deciduous and permanent human teeth.

ABSTRACT:

Introduction

Deciduous and permanent human teeth represent an excellent model system to study aging of stromal populations. Aging is tightly connected to self-renewal and proliferation and thus, mapping potential molecular differences in these characteristics between populations constitutes an important task.

Methods

Using specifically designed microarray panels, Real-Time Quantitative Polymerase Chain Reaction (RT q-PCR), Western blot, immunohistochemistry and siRNA-mediated knock down experiments, we have detected a number of molecules that were differentially expressed in dental pulp from deciduous and permanent teeth extracted from young children and adults, respectively.

Results

Among the differentially regulated genes, high-mobility group AT-hook 2 (HMGA2), a st

SUBMITTER: Kaukua N 

PROVIDER: S-EPMC4417277 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature