CD47 Enhances In Vivo Functionality of Artificial Antigen-Presenting Cells.

ABSTRACT:

Purpose

Artificial antigen-presenting cells, aAPC, have successfully been used to stimulate antigen-specific T-cell responses in vitro as well as in vivo. Although aAPC compare favorably with autologous dendritic cells in vitro, their effect in vivo might be diminished through rapid clearance by macrophages. Therefore, to prevent uptake and minimize clearance of aAPC by macrophages, thereby increasing in vivo functionality, we investigated the efficiency of "don't eat me" three-signal aAPC compared with classical two-signal aAPC.

Experimental design

To generate "don't eat me" aAPC, CD47 was additionally immobilized onto classical aAPC (aAPC(CD47+)). aAPC and aAPC(CD47+) were analyzed in in vitro human primary T-cell and macrophage cocultures. In vivo efficiency was compared

SUBMITTER: Bruns H 

PROVIDER: S-EPMC4417460 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature