3D culture broadly regulates tumor cell hypoxia response and angiogenesis via pro-inflammatory pathways.

ABSTRACT: Oxygen status and tissue dimensionality are critical determinants of tumor angiogenesis, a hallmark of cancer and an enduring target for therapeutic intervention. However, it is unclear how these microenvironmental conditions interact to promote neovascularization, due in part to a lack of comprehensive, unbiased data sets describing tumor cell gene expression as a function of oxygen levels within three-dimensional (3D) culture. Here, we utilized alginate-based, oxygen-controlled 3D tumor models to study the interdependence of culture context and the hypoxia response. Microarray gene expression analysis of tumor cells cultured in 2D versus 3D under ambient or hypoxic conditions revealed striking interdependence between culture dimensionality and hypoxia response, which was mediated in part

SUBMITTER: DelNero P 

PROVIDER: S-EPMC4417672 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature