ADAMTS-7 forms a positive feedback loop with TNF-α in the pathogenesis of osteoarthritis.

ABSTRACT:

Objective

To examine the expression of ADAMTS-7 during the progression of osteoarthritis (OA), defining its role in the pathogenesis of OA, and elucidating the molecular events involved.

Methods

ADAMTS-7 expression in cartilage of a rat OA model was assayed using immunohistochemistry. Cartilage-specific ADAMTS-7 transgenic mice and ADAMTS-7 small interfering (si)RNA knockdown mice were generated and used to analyse OA progression in both spontaneous and surgically induced OA models. Cartilage degradation and OA was evaluated using Safranin-O staining, immunohistochemistry, ELISA and western blotting. In addition, mRNA expression of tumour necrosis factor (TNF)-α and metalloproteinases known to be involved in cartilage degeneration in OA was analysed. Furthermore, the transac

SUBMITTER: Lai Y 

PROVIDER: S-EPMC4418017 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature