IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma.

ABSTRACT:

Purpose

IL2 inducible T-cell kinase (ITK) promoter CpG sites are hypomethylated in melanomas compared with nevi. The expression of ITK in melanomas, however, has not been established and requires elucidation.

Experimental design

An ITK-specific monoclonal antibody was used to probe sections from deidentified, formalin-fixed paraffin-embedded tumor blocks or cell line arrays and ITK was visualized by IHC. Levels of ITK protein differed among melanoma cell lines and representative lines were transduced with four different lentiviral constructs that each contained an shRNA designed to knockdown ITK mRNA levels. The effects of the selective ITK inhibitor BI 10N on cell lines and mouse models were also determined.

Results

ITK protein expression increased with nevus to meta

SUBMITTER: Carson CC 

PROVIDER: S-EPMC4418029 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature