A High Throughput Protein Microarray Approach to Classify HIV Monoclonal Antibodies and Variant Antigens.

ABSTRACT: In recent years, high throughput discovery of human recombinant monoclonal antibodies (mAbs) has been applied to greatly advance our understanding of the specificity, and functional activity of antibodies against HIV. Thousands of antibodies have been generated and screened in functional neutralization assays, and antibodies associated with cross-strain neutralization and passive protection in primates, have been identified. To facilitate this type of discovery, a high throughput-screening tool is needed to accurately classify mAbs, and their antigen targets. In this study, we analyzed and evaluated a prototype microarray chip comprised of the HIV-1 recombinant proteins gp140, gp120, gp41, and several membrane proximal external region peptides. The protein microarray analysis of 11 HIV-1 e

SUBMITTER: Dotsey EY 

PROVIDER: S-EPMC4418728 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature