Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy.

ABSTRACT:

Background

Grass pollen is one of the most important sources of respiratory allergies worldwide.

Objective

This study describes the development of a grass pollen allergy vaccine based on recombinant hypoallergenic derivatives of the major timothy grass pollen allergens Phl p 1, Phl p 2, Phl p 5, and Phl p 6 by using a peptide-carrier approach.

Methods

Fusion proteins consisting of nonallergenic peptides from the 4 major timothy grass pollen allergens and the PreS protein from hepatitis B virus as a carrier were expressed in Escherichia coli and purified by means of chromatography. Recombinant PreS fusion proteins were tested for allergenic activity and T-cell activation by means of IgE serology, basophil activation testing, T-cell proliferation assays, and xMAP Lumine

SUBMITTER: Focke-Tejkl M 

PROVIDER: S-EPMC4418753 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature