Miniaturized, multiplexed readout of droplet-based microfluidic assays using time-domain modulation.

ABSTRACT: Recent advances in microfluidics to generate and control picoliter emulsions of water in oil have enabled ultra-sensitive assays for small molecules, proteins, nucleic acids, and cells. Unfortunately, the conventional fluorescence detection used to measure the outcome of these droplet-based assays has not proven suited to match the time and space multiplexing capabilities of microfluidic systems. To address this challenge, we developed an in-flow fluorescence detection platform that enables multiple streams of droplets to be monitored using only a single photodetector and no lenses. The key innovation of our technology is the amplitude modulation of the signal from fluorescent droplets using distinct micro-patterned masks for each channel. By taking advantage of the high bandwidth of elect

SUBMITTER: Muluneh M 

PROVIDER: S-EPMC4418803 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature