Induced transcription and stability of CELF2 mRNA drives widespread alternative splicing during T-cell signaling.

ABSTRACT: Studies in several cell types have highlighted dramatic and diverse changes in mRNA processing that occur upon cellular stimulation. However, the mechanisms and pathways that lead to regulated changes in mRNA processing remain poorly understood. Here we demonstrate that expression of the splicing factor CELF2 (CUGBP, Elav-like family member 2) is regulated in response to T-cell signaling through combined increases in transcription and mRNA stability. Transcriptional induction occurs within 6 h of stimulation and is dependent on activation of NF-κB. Subsequently, there is an increase in the stability of the CELF2 mRNA that correlates with a change in CELF2 3'UTR length and contributes to the total signal-induced enhancement of CELF2 expression. Importantly, we uncover dozens of splicing eve

SUBMITTER: Mallory MJ 

PROVIDER: S-EPMC4418860 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature