Oxygen and glucose deprivation induces widespread alterations in mRNA translation within 20 minutes.

ABSTRACT:

Background

Oxygen and glucose metabolism play pivotal roles in many (patho)physiological conditions. In particular, oxygen and glucose deprivation (OGD) during ischemia and stroke results in extensive tissue injury and cell death.

Results

Using time-resolved ribosome profiling, we assess gene expression levels in a neural cell line, PC12, during the first hour of OGD. The most substantial alterations are seen to occur within the first 20 minutes of OGD. While transcription of only 100 genes is significantly altered during one hour of OGD, the translation response affects approximately 3,000 genes. This response involves reprogramming of initiation and elongation rates, as well as the stringency of start codon recognition. Genes involved in oxidative phosphorylation are most

SUBMITTER: Andreev DE 

PROVIDER: S-EPMC4419486 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature