Analysis of pollen-specific alternative splicing in Arabidopsis thaliana via semi-quantitative PCR.

ABSTRACT: Alternative splicing enables a single gene to produce multiple mRNA isoforms by varying splice site selection. In animals, alternative splicing of mRNA isoforms between cell types is widespread and supports cellular differentiation. In plants, at least 20% of multi-exon genes are alternatively spliced, but the extent and significance of tissue-specific splicing is less well understood, partly because it is difficult to isolate cells of a single type. Pollen is a useful model system to study tissue-specific splicing in higher plants because pollen grains contain only two cell types and can be collected in large amounts without damaging cells. Previously, we identified pollen-specific splicing patterns by comparing RNA-Seq data from Arabidopsis pollen and leaves. Here, we used semi-quantitat

SUBMITTER: Estrada AD 

PROVIDER: S-EPMC4419537 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature