3D Structural Fluctuation of IgG1 Antibody Revealed by Individual Particle Electron Tomography.

ABSTRACT: Commonly used methods for determining protein structure, including X-ray crystallography and single-particle reconstruction, often provide a single and unique three-dimensional (3D) structure. However, in these methods, the protein dynamics and flexibility/fluctuation remain mostly unknown. Here, we utilized advances in electron tomography (ET) to study the antibody flexibility and fluctuation through structural determination of individual antibody particles rather than averaging multiple antibody particles together. Through individual-particle electron tomography (IPET) 3D reconstruction from negatively-stained ET images, we obtained 120 ab-initio 3D density maps at an intermediate resolution (~1-3 nm) from 120 individual IgG1 antibody particles. Using these maps as a constraint, we deriv

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4419541 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature