Igf-I influences everolimus activity in medullary thyroid carcinoma.

ABSTRACT:

Context

Medullary thyroid carcinoma (MTC) is a rare tumor originating from thyroid parafollicular C cells. It has been previously demonstrated that insulin-like growth factor I (IGF-I) protects MTC from the effects of antiproliferative drugs. Everolimus, an mTOR inhibitor, has shown potent antiproliferative effects in a human MTC cell line, TT, and in two human MTC primary cultures.

Objective

To verify whether IGF-I may influence the effects of everolimus in a group of human MTC primary cultures.

Design

We collected 18 MTCs that were dispersed in primary cultures, treated without or with 10 nM-1 μM everolimus and/or 50 nM IGF-I. Cell viability was evaluated after 48 h, and calcitonin (CT) secretion was assessed after a 6 h incubation. IGF-I receptor downstream signali

SUBMITTER: Gentilin E 

PROVIDER: S-EPMC4419838 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature