Examining the virulence of Candida albicans transcription factor mutants using Galleria mellonella and mouse infection models.

ABSTRACT: The aim of the present study was to identify Candida albicans transcription factors (TFs) involved in virulence. Although mice are considered the gold-standard model to study fungal virulence, mini-host infection models have been increasingly used. Here, barcoded TF mutants were first screened in mice by pools of strains and fungal burdens (FBs) quantified in kidneys. Mutants of unannotated genes which generated a kidney FB significantly different from that of wild-type were selected and individually examined in Galleria mellonella. In addition, mutants that could not be detected in mice were also tested in G. mellonella. Only 25% of these mutants displayed matching phenotypes in both hosts, highlighting a significant discrepancy between the two models. To address the basis of this differe

SUBMITTER: Amorim-Vaz S 

PROVIDER: S-EPMC4419840 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature