The degradation pathway for the HBV envelope proteins involves proteolysis prior to degradation via the cytosolic proteasome.

ABSTRACT: To study the pathway of degradation of the hepatitis B virus (HBV) middle envelope protein (M), human hepatoblastoma cells were transfected with a plasmid that specifies production of M in the absence of other viral proteins. When expressed in HepG2 cells, 90% of M protein was secreted into the culture media within a 24-h period. However, quite surprisingly, 10% of this protein remained cell associated and was only slowly degraded over a 24-48-h period. Treatment with inhibitors of the cytosolic proteasome complex resulted in the accumulation of full-length HBV M protein and M derived HBV-specific polypeptides of 20 and 17 kDa. Treatment with the endoglycosidases PNGase F and Endo H, confirmed that the two species were derived from a similar polypeptide with a N-linked glycan modification.

SUBMITTER: Liu Y 

PROVIDER: S-EPMC4422056 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature