Whole genome sequence comparison of ten diagnostic brucellaphages propagated on two Brucella abortus hosts.

ABSTRACT:

Background

Recently the genome sequences of two brucellaphages, isolated in Georgia (Tb) and Mexico (Pr) were reported revealing pronounced sequence homogeneity and the presence of two major indels discriminating the two phages. Subsequent genome sequencing of six diagnostic brucellaphages: Tbilisi (Tb), Firenze (Fz), Weybridge (Wb), S708, Berkeley (Bk) and R/C phages identified three major genetic groups. However, the propensity for fine-scale genetic variability of diverse brucellaphages grown on multiple hosts within a single Brucella species remains unknown.

Methods

We sequenced the complete genomes of ten brucellaphages following initial propagation on B. abortus strain 141 and after subsequent propagation on B. abortus strain S19. All brucellaphages were isolated and p

SUBMITTER: Tevdoradze E 

PROVIDER: S-EPMC4422536 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature