Redesigning the regulatory pathway to enhance cellulase production in Penicillium oxalicum.

ABSTRACT:

Background

In cellulolytic fungi, induction and repression mechanisms synchronously regulate the synthesis of cellulolytic enzymes for accurate responses to carbon sources in the environment. Many proteins, particularly transcription regulatory factors involved in these processes, were identified and genetically engineered in Penicillium oxalicum and other cellulolytic fungi. Despite such great efforts, its effect of modifying a single target to improve the production of cellulase is highly limited.

Results

In this study, we developed a systematic strategy for the genetic engineering of P. oxalicum to enhance cellulase yields, by enhancing induction (by blocking intracellular inducer hydrolysis and increasing the activator level) and relieving the repression. We obtained a t

SUBMITTER: Yao G 

PROVIDER: S-EPMC4422585 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature