Dissecting the calcium-induced differentiation of human primary keratinocytes stem cells by integrative and structural network analyses.

ABSTRACT: The molecular details underlying the time-dependent assembly of protein complexes in cellular networks, such as those that occur during differentiation, are largely unexplored. Focusing on the calcium-induced differentiation of primary human keratinocytes as a model system for a major cellular reorganization process, we look at the expression of genes whose products are involved in manually-annotated protein complexes. Clustering analyses revealed only moderate co-expression of functionally related proteins during differentiation. However, when we looked at protein complexes, we found that the majority (55%) are composed of non-dynamic and dynamic gene products ('di-chromatic'), 19% are non-dynamic, and 26% only dynamic. Considering three-dimensional protein structures to predict steric in

SUBMITTER: Toufighi K 

PROVIDER: S-EPMC4422705 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature