MPP1 as a Factor Regulating Phase Separation in Giant Plasma Membrane-Derived Vesicles.

ABSTRACT: The existence of membrane-rafts helps to conceptually understand the spatiotemporal organization of membrane-associated events (signaling, fusion, fission, etc.). However, as rafts themselves are nanoscopic, dynamic, and transient assemblies, they cannot be directly observed in a metabolizing cell by traditional microscopy. The observation of phase separation in giant plasma membrane-derived vesicles from live cells is a powerful tool for studying lateral heterogeneity in eukaryotic cell membranes, specifically in the context of membrane rafts. Microscopic phase separation is detectable by fluorescent labeling, followed by cooling of the membranes below their miscibility phase transition temperature. It remains unclear, however, if this lipid-driven process is tuneable in any way by intera

SUBMITTER: Podkalicka J 

PROVIDER: S-EPMC4423064 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature