Dual-color dual-focus line-scanning FCS for quantitative analysis of receptor-ligand interactions in living specimens.

ABSTRACT: Cellular communication in multi-cellular organisms is mediated to a large extent by a multitude of cell-surface receptors that bind specific ligands. An in-depth understanding of cell signaling networks requires quantitative information on ligand-receptor interactions within living systems. In principle, fluorescence correlation spectroscopy (FCS) based methods can provide such data, but live-cell applications have proven extremely challenging. Here, we have developed an integrated dual-color dual-focus line-scanning fluorescence correlation spectroscopy (2c2f lsFCS) technique that greatly facilitates live-cell and tissue experiments. Absolute ligand and receptor concentrations and their diffusion coefficients within the cell membrane can be quantified without the need to perform additiona

SUBMITTER: Dorlich RM 

PROVIDER: S-EPMC4423563 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature