Engineered baker's yeast as whole-cell biocatalyst for one-pot stereo-selective conversion of amines to alcohols.

ABSTRACT:

Background

One-pot multi-step biocatalysis is advantageous over step-by-step synthesis as it reduces the number of process operation units, leading to significant process intensification. Whole-cell biocatalysis with metabolically active cells is especially valuable since all enzymes can be co-expressed in the cell whose metabolism can be exploited for supply of co-substrates and co-factors.

Results

In this study, a heterologous enzymatic system consisting of ω-transaminase and ketone reductase was introduced in Saccharomyces cerevisiae, and evaluated for one-pot stereo-selective conversion of amines to alcohols. The system was applied for simultaneous kinetic resolution of racemic 1-phenylethylamine to (R)-1-phenylethylamine and reduction of the ketone intermediate to (R)-1

SUBMITTER: Weber N 

PROVIDER: S-EPMC4423645 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature