Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.

ABSTRACT: Renewable in vitro cell cultures, such as lymphoblastoid cell lines (LCLs), have facilitated studies that contributed to our understanding of genetic influence on human traits. However, the degree to which cell lines faithfully maintain differences in donor-specific phenotypes is still debated. We have previously reported that standard cell line maintenance practice results in a loss of donor-specific gene expression signatures in LCLs. An alternative to the LCL model is the induced pluripotent stem cell (iPSC) system, which carries the potential to model tissue-specific physiology through the use of differentiation protocols. Still, existing LCL banks represent an important source of starting material for iPSC generation, and it is possible that the disruptions in gene regulation associat

SUBMITTER: Thomas SM 

PROVIDER: S-EPMC4423863 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature