Spectral fingerprinting of individual cells visualized by cavity-reflection-enhanced light-absorption microscopy.

ABSTRACT: The absorption spectrum of light is known to be a "molecular fingerprint" that enables analysis of the molecular type and its amount. It would be useful to measure the absorption spectrum in single cell in order to investigate the cellular status. However, cells are too thin for their absorption spectrum to be measured. In this study, we developed an optical-cavity-enhanced absorption spectroscopic microscopy method for two-dimensional absorption imaging. The light absorption is enhanced by an optical cavity system, which allows the detection of the absorption spectrum with samples having an optical path length as small as 10 μm, at a subcellular spatial resolution. Principal component analysis of various types of cultured mammalian cells indicates absorption-based cellular diversity. Inte

SUBMITTER: Arai Y 

PROVIDER: S-EPMC4423951 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature