A 190 base pair, TGF-β responsive tooth and fin enhancer is required for stickleback Bmp6 expression.

ABSTRACT: The ligands of the Bone Morphogenetic Protein (BMP) family of developmental signaling molecules are often under the control of complex cis-regulatory modules and play diverse roles in vertebrate development and evolution. Here, we investigated the cis-regulatory control of stickleback Bmp6. We identified a 190bp enhancer ~2.5 kilobases 5' of the Bmp6 gene that recapitulates expression in developing teeth and fins, with a core 72bp sequence that is sufficient for both domains. By testing orthologous enhancers with varying degrees of sequence conservation from outgroup teleosts in transgenic reporter gene assays in sticklebacks and zebrafish, we found that the function of this regulatory element appears to have been conserved for over 250 million years of teleost evolution. We show that a pr

SUBMITTER: Erickson PA 

PROVIDER: S-EPMC4424077 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature