Quantitative proteogenomic profiling of epidermal barrier formation in vitro.

ABSTRACT:

Background

The barrier function of the epidermis is integral to personal well-being, and defects in the skin barrier are associated with several widespread diseases. Currently there is a limited understanding of system-level proteomic changes during epidermal stratification and barrier establishment.

Objective

Here we report the quantitative proteogenomic profile of an in vitro reconstituted epidermis at three time points of development in order to characterize protein changes during stratification.

Methods

The proteome was measured using data-dependent "shotgun" mass spectrometry and quantified with statistically validated label-free proteomic methods for 20 replicates at each of three time points during the course of epidermal development.

Results

Over 3600 p

SUBMITTER: Winget JM 

PROVIDER: S-EPMC4424144 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature