The dynamics of plus end polarization and microtubule assembly during Xenopus cortical rotation.

ABSTRACT: The self-organization of dorsally-directed microtubules during cortical rotation in the Xenopus egg is essential for dorsal axis formation. The mechanisms controlling this process have been problematic to analyze, owing to difficulties in visualizing microtubules in living egg. Also, the order of events occurring at the onset of cortical rotation have not been satisfactorily visualized in vivo and have been inferred from staged fixed samples. To address these issues, we have characterized the dynamics of total microtubule and plus end behavior continuously throughout cortical rotation, as well as in oocytes and unfertilized eggs. Here, we show that the nascent microtubule network forms in the cortex but associates with the deep cytoplasm at the start of rotation. Importantly, plus ends rem

SUBMITTER: Olson DJ 

PROVIDER: S-EPMC4424176 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature