Inorganic polyphosphate elicits pro-inflammatory responses through activation of the mammalian target of rapamycin complexes 1 and 2 in vascular endothelial cells.

ABSTRACT:

Background

Inorganic polyphosphate (polyP) elicits pro-inflammatory signaling responses in endothelial cells through interaction with two receptors, RAGE and P2Y1 . It is known that polyP activates mTOR signaling in breast cancer cells.

Objectives

The objective of this study is to understand the mechanism of the polyP-mediated signaling pathway in endothelial cells and to determine whether polyP exerts its pro-inflammatory effect through activation of mTOR.

Methods

mTOR activation by polyP or platelet releasates in cellular and animal models was monitored in the absence and presence of pharmacological inhibitors and/or siRNA knockdown of specific signaling molecules.

Results

PolyP effectively induced phosphorylation of mTOR complex 1 (mTORC1) substrate, p70S6K,

SUBMITTER: Hassanian SM 

PROVIDER: S-EPMC4424178 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature