Quantitative proteome analysis of temporally resolved phagosomes following uptake via key phagocytic receptors.

ABSTRACT: Macrophages operate at the forefront of innate immunity and their discrimination of foreign versus "self" particles is critical for a number of responses including efficient pathogen killing, antigen presentation, and cytokine induction. In order to efficiently destroy the particles and detect potential threats, macrophages express an array of receptors to sense and phagocytose prey particles. In this study, we accurately quantified a proteomic time-course of isolated phagosomes from murine bone marrow-derived macrophages induced by particles conjugated to seven different ligands representing pathogen-associated molecular patterns, immune opsonins or apoptotic cell markers. We identified a clear functional differentiation over the three timepoints and detected subtle differences between ce

SUBMITTER: Dill BD 

PROVIDER: S-EPMC4424403 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature