Quantum dot assisted tracking of the intracellular protein Cyclin E in Xenopus laevis embryos.

ABSTRACT:

Background

Luminescent semiconductor nanocrystals, also known as quantum dots (QD), possess highly desirable optical properties that account for development of a variety of exciting biomedical techniques. These properties include long-term stability, brightness, narrow emission spectra, size tunable properties and resistance to photobleaching. QD have many promising applications in biology and the list is constantly growing. These applications include DNA or protein tagging for in vitro assays, deep-tissue imaging, fluorescence resonance energy transfer (FRET), and studying dynamics of cell surface receptors, among others. Here we explored the potential of QD-mediated labeling for the purpose of tracking an intracellular protein inside live cells.

Results

We manufactured dih

SUBMITTER: Brandt YI 

PROVIDER: S-EPMC4424550 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature