Effect of influenza A(H5N1) vaccine prepandemic priming on CD4+ T-cell responses.

ABSTRACT:

Introduction

Previous priming with avian influenza vaccines results in more rapid and more robust neutralizing antibody responses upon revaccination, but the role CD4(+) T cells play in this process is not currently known.

Methods

Human subjects previously enrolled in trials of inactivated influenza A(H5N1) vaccines and naive subjects were immunized with an inactivated subunit influenza A/Indonesia/5/05(H5N1) vaccine. Neutralizing antibody responses were measured by a microneutralization assay, and hemagglutinin (HA)-specific and nucleoprotein (NP)-specific CD4(+) T-cell responses were quantified using interferon γ enzyme-linked immunosorbent spot assays.

Results

While vaccination induced barely detectable CD4(+) T-cell responses specific for HA in the previously unpr

SUBMITTER: Nayak JL 

PROVIDER: S-EPMC4425838 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature