Genome-wide mapping of histone H3 lysine 4 trimethylation in Eucalyptus grandis developing xylem.

ABSTRACT:

Background

Histone modifications play an integral role in plant development, but have been poorly studied in woody plants. Investigating chromatin organization in wood-forming tissue and its role in regulating gene expression allows us to understand the mechanisms underlying cellular differentiation during xylogenesis (wood formation) and identify novel functional regions in plant genomes. However, woody tissue poses unique challenges for using high-throughput chromatin immunoprecipitation (ChIP) techniques for studying genome-wide histone modifications in vivo. We investigated the role of the modified histone H3K4me3 (trimethylated lysine 4 of histone H3) in gene expression during the early stages of wood formation using ChIP-seq in Eucalyptus grandis, a woody biomass model.

Re

SUBMITTER: Hussey SG 

PROVIDER: S-EPMC4425858 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature