Construction of a virtual Mycobacterium tuberculosis consensus genome and its application to data from a next generation sequencer.

ABSTRACT:

Background

Although Mycobacterium tuberculosis isolates are consisted of several different lineages and the epidemiology analyses are usually assessed relative to a particular reference genome, M. tuberculosis H37Rv, which might introduce some biased results. Those analyses are essentially based genome sequence information of M. tuberculosis and could be performed in sillico in theory, with whole genome sequence (WGS) data available in the databases and obtained by next generation sequencers (NGSs). As an approach to establish higher resolution methods for such analyses, whole genome sequences of the M. tuberculosis complexes (MTBCs) strains available on databases were aligned to construct virtual reference genome sequences called the consensus sequence (CS), and evaluated its feas

SUBMITTER: Okumura K 

PROVIDER: S-EPMC4425900 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature